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30f11  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec 30f11
    Antibodies for immunofluorescence staining of mouse spinal cord tissue and human tissue.
    30f11, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 350 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd45+antibody/CD45+Antibody%2C+anti-mouse/pmc13693491-14-4-6
    Average 96 stars, based on 350 article reviews
    30f11 - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "Astrocytic EAAT ‐2 Upregulation Supports Neuro‐Axonal Homeostasis in Chronic Inflammatory CNS Lesions"

    Article Title: Astrocytic EAAT ‐2 Upregulation Supports Neuro‐Axonal Homeostasis in Chronic Inflammatory CNS Lesions

    Journal: Glia

    doi: 10.1002/glia.70243

    Antibodies for immunofluorescence staining of mouse spinal cord tissue and human tissue.
    Figure Legend Snippet: Antibodies for immunofluorescence staining of mouse spinal cord tissue and human tissue.

    Techniques Used: Immunofluorescence, Staining, Tissue

    Related Articles

    Staining:

    Article Title: Integrative Multiomic Classification Reveals Distinct Origins and Evolutionary Trajectories of Thymic Epithelial Tumors
    Article Snippet: Then, red blood cell lysis was done by treating 2 mL ACK lysis buffer (Gibco, #A1049201) for 5 minutes at room temperature, followed by PBS washing. .. Leukocytes were stained with CD45 antibody (Miltenyi Biotec, #130-045-801, RRID: AB_2783001) and depleted with autoMACS Pro Separator (Miltenyi Biotec). .. After washing, a single-cell RNA-seq library was generated using Chromium Single Cell 3′ Library and Gel Bead Kit v2 (10x Genomics).

    Article Title: Iron diminishes immunosuppressive macrophages and enhances anti-PD-1 immunotherapy in breast cancer models.
    Article Snippet: Following Ficoll separation, dead cells were removed by using dead cell removal kit (Cat#130–090–101, Miltenyi Biotec) per manufacturer’s instruction. .. The live cell fraction was blocked by incubation with TruStain FcX in 50 μL cell staining buffer for 20 min on ice, then incubated with magnetically labeled CD45 antibody (Cat#130–052–301, Miltenyi Biotec) and passed through a LS magnetic column (Cat#130–042– 401, Miltenyi Biotec). ..

    Article Title: Integrative Multi-omic Classification Reveals Distinct Origins and Evolutionary Trajectories of Thymic Epithelial Tumors
    Article Snippet: Then, RBC lysis was done by treating 2ml ACK lysis buffer (Gibco #A1049201) for 5 minutes at room temperature, followed by PBS washing. .. Leukocytes were stained with CD45 antibody (Miltenyi Biotec #130-045-801, RRID: AB_2783001) and depleted with autoMACS Pro Separator (Miltenyi Biotec). .. After washing, a single-cell RNA sequencing library was generated using Chromium Single Cell 3’ Library & Gel Bead Kit v2 (10x Genomics).

    Article Title: Iron diminishes immunosuppressive macrophages and enhances anti-PD-1 immunotherapy in breast cancer models
    Article Snippet: Following Ficoll separation, dead cells were removed by using dead cell removal kit (Cat#130–090–101, Miltenyi Biotec) per manufacturer’s instruction. .. The live cell fraction was blocked by incubation with TruStain FcX in 50 μL cell staining buffer for 20 min on ice, then incubated with magnetically labeled CD45 antibody (Cat#130–052–301, Miltenyi Biotec) and passed through a LS magnetic column (Cat#130–042–401, Miltenyi Biotec). ..

    other:

    Article Title: Protocol for isolating stromal cells from lymphoid tissue for performing scRNA-seq
    Article Snippet: CD45 antibody, anti-mouse, Biotin (Dilutions in 1:50) , Miltenyi Biotec , Cat# 130-124-209, RRID: AB_2819580.

    Article Title: Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing
    Article Snippet: CD45 antibody, anti-human, Vio R667, REAfinity , Miltenyi Biotec B.V. & Co. KG , Cat# 130-128-742 RRID: AB_2905022.

    Article Title: Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing
    Article Snippet: CD45 antibody, anti-human, FITC, REAfinity , Miltenyi Biotec B.V. & Co. KG , Cat# 130-110-631 RRID: AB_2658237.

    Incubation:

    Article Title: Iron diminishes immunosuppressive macrophages and enhances anti-PD-1 immunotherapy in breast cancer models.
    Article Snippet: Following Ficoll separation, dead cells were removed by using dead cell removal kit (Cat#130–090–101, Miltenyi Biotec) per manufacturer’s instruction. .. The live cell fraction was blocked by incubation with TruStain FcX in 50 μL cell staining buffer for 20 min on ice, then incubated with magnetically labeled CD45 antibody (Cat#130–052–301, Miltenyi Biotec) and passed through a LS magnetic column (Cat#130–042– 401, Miltenyi Biotec). ..

    Article Title: Iron diminishes immunosuppressive macrophages and enhances anti-PD-1 immunotherapy in breast cancer models
    Article Snippet: Following Ficoll separation, dead cells were removed by using dead cell removal kit (Cat#130–090–101, Miltenyi Biotec) per manufacturer’s instruction. .. The live cell fraction was blocked by incubation with TruStain FcX in 50 μL cell staining buffer for 20 min on ice, then incubated with magnetically labeled CD45 antibody (Cat#130–052–301, Miltenyi Biotec) and passed through a LS magnetic column (Cat#130–042–401, Miltenyi Biotec). ..

    Labeling:

    Article Title: Iron diminishes immunosuppressive macrophages and enhances anti-PD-1 immunotherapy in breast cancer models.
    Article Snippet: Following Ficoll separation, dead cells were removed by using dead cell removal kit (Cat#130–090–101, Miltenyi Biotec) per manufacturer’s instruction. .. The live cell fraction was blocked by incubation with TruStain FcX in 50 μL cell staining buffer for 20 min on ice, then incubated with magnetically labeled CD45 antibody (Cat#130–052–301, Miltenyi Biotec) and passed through a LS magnetic column (Cat#130–042– 401, Miltenyi Biotec). ..

    Article Title: Iron diminishes immunosuppressive macrophages and enhances anti-PD-1 immunotherapy in breast cancer models
    Article Snippet: Following Ficoll separation, dead cells were removed by using dead cell removal kit (Cat#130–090–101, Miltenyi Biotec) per manufacturer’s instruction. .. The live cell fraction was blocked by incubation with TruStain FcX in 50 μL cell staining buffer for 20 min on ice, then incubated with magnetically labeled CD45 antibody (Cat#130–052–301, Miltenyi Biotec) and passed through a LS magnetic column (Cat#130–042–401, Miltenyi Biotec). ..



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    Miltenyi Biotec cd45 pe
    Experimental Design and Characterization of FAPs and Freshly Isolated MVFs. (A) Schematic representing the study design (Created with BioRender.com). (B) Representative phase contrast image of a single microvascular fragment immediately after isolation. (C) Representative flow cytometry gating strategy and scatterplots of PDGFRα and SCA‐1 expression in FAPs after magnetic‐activated cell sorting.
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    Image Search Results


    Antibodies for immunofluorescence staining of mouse spinal cord tissue and human tissue.

    Journal: Glia

    Article Title: Astrocytic EAAT ‐2 Upregulation Supports Neuro‐Axonal Homeostasis in Chronic Inflammatory CNS Lesions

    doi: 10.1002/glia.70243

    Figure Lengend Snippet: Antibodies for immunofluorescence staining of mouse spinal cord tissue and human tissue.

    Article Snippet: CD45 , PE , 30F11 , Miltenyi Biotec , 130‐117‐498 , Rat , ms , 1:20.

    Techniques: Immunofluorescence, Staining, Tissue

    Experimental Design and Characterization of FAPs and Freshly Isolated MVFs. (A) Schematic representing the study design (Created with BioRender.com). (B) Representative phase contrast image of a single microvascular fragment immediately after isolation. (C) Representative flow cytometry gating strategy and scatterplots of PDGFRα and SCA‐1 expression in FAPs after magnetic‐activated cell sorting.

    Journal: Advanced Biology

    Article Title: Fibro‐Adipogenic Progenitors From Skeletal Muscle Enhance Microvascular Angiogenesis in a 3D Model

    doi: 10.1002/adbi.70162

    Figure Lengend Snippet: Experimental Design and Characterization of FAPs and Freshly Isolated MVFs. (A) Schematic representing the study design (Created with BioRender.com). (B) Representative phase contrast image of a single microvascular fragment immediately after isolation. (C) Representative flow cytometry gating strategy and scatterplots of PDGFRα and SCA‐1 expression in FAPs after magnetic‐activated cell sorting.

    Article Snippet: Briefly, the adherent cells were resuspended in 200 μL of DMEM + 2% FBS and incubated with antibodies (1 μL per 2.0 × 10 6 cells) against PDGFRα (ab203491, Abcam), SCA‐1 (AB4336, Millipore Sigma), CD31‐PE (130‐116‐538, Miltenyi Biotec), and CD45‐PE (130‐126‐312, Miltenyi Biotec) on ice for 30 min. For CD31/CD45 depletion, cells were incubated with 10 μL of Anti‐PE MicroBeads (130‐048‐801, Miltenyi Biotec) on ice for 30 min, washed twice in 1 mL of MACS buffer + 0.5% bovine serum albumin (BSA) via centrifugation (2000 rpm × 3 min at 4°C), and subjected to negative selection with LD Columns (130‐042‐901, Miltenyi Biotec) according to manufacturer protocols.

    Techniques: Isolation, Phase Contrast, Flow Cytometry, Expressing, FACS, Co-Cultures, Confocal Microscopy